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hpi2 pe cy7  (Novus Biologicals)


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    Novus Biologicals hpi2 pe cy7
    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells <t>(HPi2+GFP+NTPDase3+)</t> from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.
    Hpi2 Pe Cy7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbp1+18946pecy7/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BPE%2FCy7%5D/pm37943614-212-7-8
    Average 92 stars, based on 3 article reviews
    hpi2 pe cy7 - by Bioz Stars, 2026-11
    92/100 stars

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    1) Product Images from "HNF1α maintains pancreatic α and β cell functions in primary human islets."

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.

    Journal: JCI insight

    doi: 10.1172/jci.insight.170884

    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.
    Figure Legend Snippet: Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.

    Techniques Used: RNA Sequencing, Isolation, Control, Expressing, Quantitative Proteomics

    Figure 4. RNA-Seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA-Seq (n = 4 donors); left image is bright-field, and right image is blue light (488 nm) of human HNF1AKD pseudoislets. Scale bars: 1,000 μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) DEG analysis revealed significantly up- and downregulated genes after HNF1AKD in α cells. FC = 1.5, adjusted P = 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-spe- cific consequences of HNF1AKD. (E and F) Gene Ontology (GO) pathways of shared (E) and α cell (F) enriched DEG sets. (G) Box plots displaying TPM of select DEGs. *P < 0.05.
    Figure Legend Snippet: Figure 4. RNA-Seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA-Seq (n = 4 donors); left image is bright-field, and right image is blue light (488 nm) of human HNF1AKD pseudoislets. Scale bars: 1,000 μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) DEG analysis revealed significantly up- and downregulated genes after HNF1AKD in α cells. FC = 1.5, adjusted P = 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-spe- cific consequences of HNF1AKD. (E and F) Gene Ontology (GO) pathways of shared (E) and α cell (F) enriched DEG sets. (G) Box plots displaying TPM of select DEGs. *P < 0.05.

    Techniques Used: RNA Sequencing, Isolation, Control

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    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells <t>(HPi2+GFP+NTPDase3+)</t> from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.
    Hpi2 Pe Cy7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbp1+18946pecy7/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BPE%2FCy7%5D/pm37943614-212-7-8
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    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells <t>(HPi2+GFP+NTPDase3+)</t> from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.
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    https://www.bioz.com/product/nbp1+18946pecy7/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BPE%2FCy7%5D/pm36634673-457-13-16
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    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells <t>(HPi2+GFP+NTPDase3+)</t> from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.
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    Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.

    Journal: JCI insight

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.

    doi: 10.1172/jci.insight.170884

    Figure Lengend Snippet: Figure 3. RNA-Seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-to-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseu- doislets for downstream RNA-Seq (n = 4 donors). (B) Fraction of endocrine (HPi2+) cells expressing GFP in sorted samples. (C) HNF1A transcripts per million (TPM) in sequenced samples. (D) Differential expression analysis revealed significantly up- and downregulated genes after HNF1AKD in β cells. Fold change (FC) = 1.5, adjusted P = 0.05. (E) Heatmap of DEGs in β cells after HNF1AKD. (F–H) Significantly downregulated (F) and upregulated (G) Gene Ontology (GO) pathways and downregulated KEGG pathways (H) in HNF1AKD relative to control β cells. *P < 0.05.

    Article Snippet: The following primary-secondary conjugated antibodies were used: HPi2-PE/Cy7 (Novus Biologicals NBP1-18946PECY7), NTPDase3-647 (Jean Sévigny’s lab clone hN3-B3S), and CD26-PE (BioLegend 302706) (Supplemental Table 2).

    Techniques: RNA Sequencing, Isolation, Control, Expressing, Quantitative Proteomics

    Figure 4. RNA-Seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA-Seq (n = 4 donors); left image is bright-field, and right image is blue light (488 nm) of human HNF1AKD pseudoislets. Scale bars: 1,000 μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) DEG analysis revealed significantly up- and downregulated genes after HNF1AKD in α cells. FC = 1.5, adjusted P = 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-spe- cific consequences of HNF1AKD. (E and F) Gene Ontology (GO) pathways of shared (E) and α cell (F) enriched DEG sets. (G) Box plots displaying TPM of select DEGs. *P < 0.05.

    Journal: JCI insight

    Article Title: HNF1α maintains pancreatic α and β cell functions in primary human islets.

    doi: 10.1172/jci.insight.170884

    Figure Lengend Snippet: Figure 4. RNA-Seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA-Seq (n = 4 donors); left image is bright-field, and right image is blue light (488 nm) of human HNF1AKD pseudoislets. Scale bars: 1,000 μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) DEG analysis revealed significantly up- and downregulated genes after HNF1AKD in α cells. FC = 1.5, adjusted P = 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-spe- cific consequences of HNF1AKD. (E and F) Gene Ontology (GO) pathways of shared (E) and α cell (F) enriched DEG sets. (G) Box plots displaying TPM of select DEGs. *P < 0.05.

    Article Snippet: The following primary-secondary conjugated antibodies were used: HPi2-PE/Cy7 (Novus Biologicals NBP1-18946PECY7), NTPDase3-647 (Jean Sévigny’s lab clone hN3-B3S), and CD26-PE (BioLegend 302706) (Supplemental Table 2).

    Techniques: RNA Sequencing, Isolation, Control